Evaluation of correlation between NF-kappa B mediated PAI-1 gene and sepsis

科研成果详情

题名	Evaluation of correlation between NF-kappa B mediated PAI-1 gene and sepsis
作者	Wang, L. -X.1,2; Tang, L. -M.2,3; Pan, G. -Q.1,2; Yan, C. -X.1,2; Lu, L.1,2; Liang, Y. -F.1,2
发表日期	2017-01
发表期刊	EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES 影响因子和分区
语种	英语
原始文献类型	Review
关键词	NF-kappa B signaling pathway PAI-1 gene Sepsis Correlation Promotion
其他关键词	PULMONARY-FIBROSIS ; PATHWAY ; CELLS ; ATHEROSCLEROSIS ; ACTIVATION ; GAMMA
摘要	OBJECTIVE: The present study is aimed to explore the correlation between PAI-1 gene mediated by NF-kappa B signaling pathway and human sepsis. PATIENTS AND METHODS: In this study, we used 74 cases of sepsis patients preserved by the laboratory as the observation group, and 68 cases of healthy people served the control group. Further methods like fluorescence quantitative PCR, enzyme-linked immunosorbent assay, Western- blotting were used to determine NF-B expression, NF-kappa B gene mRNA analyses and protein expression on different research subjects. Further, the positive expression rates of PAI-1 gene in the observation group and the control group were determined by immunohistochemistry. RESULTS: The expression levels of NF-.B and PAI-1 gene mRNA in the blood of the observation group significantly increased in comparison to control group (X2 = 3.24, p < 0.05; X2 = 2.81, p < 0.05). Also, NF-kappa F and PAI-1 gene protein expressions (0.14 ug/l, 0.32 ug/l) were significantly higher in the observation group in comparison to control group (p < 0.05). The results of blood glucose measurement showed that the fasting blood glucose (14.3 mmol/l) in the observation group was significantly higher than that in the control group (4.6 mmol/l). Immunohistochemical were also in sync with above results. CONCLUSIONS: The present study concludes that PAI-1 gene expression gets significantly increased via NF-kappa F signaling pathway during sepsis.
出版者	VERDUCI PUBLISHER
出版地	ROME
ISSN	1128-3602
卷号	21 期号:1 页码:30-36
页数	7
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:000395656100005
收录类别	SCIE
PubMed ID	2812136
通讯作者地址	[Tang, L. -M.]Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou, Peoples R China. ; [Tang, L. -M.]Wenzhou Med Univ, Affiliated Hosp 2, Dept Emergency, Wenzhou, Peoples R China.
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/12304
专题	附属第二医院_儿童ICU 附属第二医院第二临床医学院，附属第二医院、育英儿童医院
通讯作者	Tang, L. -M.
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 2, Dept Pediat ICU, Wenzhou, Peoples R China; 2.Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou, Peoples R China; 3.Wenzhou Med Univ, Affiliated Hosp 2, Dept Emergency, Wenzhou, Peoples R China
第一作者单位	附属第二医院_儿童ICU; 第二临床医学院，附属第二医院、育英儿童医院
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院
第一作者的第一单位	附属第二医院_儿童ICU
推荐引用方式 GB/T 7714	Wang, L. -X.,Tang, L. -M.,Pan, G. -Q.,et al. Evaluation of correlation between NF-kappa B mediated PAI-1 gene and sepsis[J]. EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,2017,21(1):30-36.
APA	Wang, L. -X., Tang, L. -M., Pan, G. -Q., Yan, C. -X., Lu, L., & Liang, Y. -F.. (2017). Evaluation of correlation between NF-kappa B mediated PAI-1 gene and sepsis. EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 21(1), 30-36.
MLA	Wang, L. -X.,et al."Evaluation of correlation between NF-kappa B mediated PAI-1 gene and sepsis".EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES 21.1(2017):30-36.