miR-340-FHL2 axis inhibits cell growth and metastasis in ovarian cancer

doi:10.1038/s41419-019-1604-3

科研成果详情

题名	miR-340-FHL2 axis inhibits cell growth and metastasis in ovarian cancer
作者	Huang, Zheng 1; Li, Qiuxia1; Luo, Kaili 1; Zhang, Qinkai 1; Geng, Jingwen 1; Zhou, Xunzhu 1; Xu, Yesha1; Qian, Mengyao 1; Zhang, Jian-an2; Ji, Liying1; Wu, Jianmin1
发表日期	2019-05-08
发表期刊	CELL DEATH & DISEASE 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	EPITHELIAL-MESENCHYMAL TRANSITION ; PROTEIN FHL2 ; SCAFFOLD PROTEIN ; DNA METHYLATION ; BETA-CATENIN ; TUMOR-GROWTH ; EXPRESSION ; INTERACTS ; MICRORNAS ; DIFFERENTIATION
摘要	Although increasing evidence indicated that deregulation of microRNAs (miRNAs) contributed to tumor initiation and progression, but little is known about the biological role of miR-340 in ovarian cancer (OC). In this study, we found that miR-340 expression was downregulated in OC tissues compared with its expression in normal ovarian epithelium and endometrium, and treatment with 5-aza-2'-deoxycytidine (5-Aza-dC) or trichostatin A (TSA) increased miR-340 expression in OC cells. In addition, ectopic miR-340 expression inhibited OC cell growth and metastasis in vitro and in vivo. Four and a half LIM domains protein 2 (FHL2) was confirmed as a direct target of miR-340 and silencing FHL2 mimicked the effects of miR-340 in OC cells. Further mechanistic study showed that miR-340 inhibited the Wnt/beta-catenin pathway by targeting FHL2, as well as downstream cell cycle and epithelial-to-mesenchymal transition (EMT) signals in OC cells. Moreover, the greatest association between miR-340 and FHL2 was found in 481 ovarian serous cystadenocarcinoma tissues via pan-cancer analysis. Finally, we revealed that lower miR-340 or higher FHL2 was associated with poor OC patient outcomes. Our findings indicate that the miR-340-FHL2 axis regulates Wnt/beta-catenin signaling and is involved in tumorigenesis in OC. Therefore, manipulating the expression of miR-340 or its target genes is a potential strategy in OC therapy.
资助项目	National Natural Sciences Foundation of ChinaNational Natural Science Foundation of China (NSFC) [NSFC-81472651, 81201589]
出版者	NATURE PUBLISHING GROUP
出版地	LONDON
ISSN	2041-4889
卷号	10 期号:5 页码:372
DOI	10.1038/s41419-019-1604-3
页数	16
WOS类目	Cell Biology
WOS研究方向	Cell Biology
WOS记录号	WOS:000468670100006
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	31068580
PMC记录号	PMC6506554
SCOPUSEID	2-s2.0-85065442875
通讯作者地址	[Wu, Jianmin]Institute of Genomic Medicine,Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Immunology;Cellular and Molecular Neuroscience;Cell Biology;Cancer Research
TOP期刊	TOP期刊
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/12258
专题	基因组医学研究院附属第二医院第二临床医学院、附属第二医院、育英儿童医院_妇产科学附属第二医院_妇产科
通讯作者	Wu, Jianmin
作者单位	1.Institute of Genomic Medicine,Wenzhou Medical University,Wenzhou,325000,China; 2.Department of Obstetrics and Gynecology,The Second Affiliated Hospital and Yuying Children of Wenzhou Medical University,Wenzhou,325027,China
第一作者单位	基因组医学研究院
通讯作者单位	基因组医学研究院
第一作者的第一单位	基因组医学研究院
推荐引用方式 GB/T 7714	Huang, Zheng,Li, Qiuxia,Luo, Kaili,et al. miR-340-FHL2 axis inhibits cell growth and metastasis in ovarian cancer[J]. CELL DEATH & DISEASE,2019,10(5):372.
APA	Huang, Zheng., Li, Qiuxia., Luo, Kaili., Zhang, Qinkai., Geng, Jingwen., ... & Wu, Jianmin. (2019). miR-340-FHL2 axis inhibits cell growth and metastasis in ovarian cancer. CELL DEATH & DISEASE, 10(5), 372.
MLA	Huang, Zheng,et al."miR-340-FHL2 axis inhibits cell growth and metastasis in ovarian cancer".CELL DEATH & DISEASE 10.5(2019):372.