Potentially predictive microRNAs of gastric cancer with metastasis to lymph node

doi:10.3748/wjg.v17.i31.3645

科研成果详情

题名	Potentially predictive microRNAs of gastric cancer with metastasis to lymph node
作者	Wu, Wen-Yi1; Xue, Xiang-Yang2; Chen, Zhe-Jing1; Han, Shao-Liang1; Huang, Ying-Peng1; Zhang, Li-Fang 2; Zhu, Guan-Bao1; Shen, Xian1
发表日期	2011-08-21
发表期刊	WORLD JOURNAL OF GASTROENTEROLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	MicroRNA miR-212 MiR-195 Gastric cancer Metastasis to lymph node
其他关键词	EXPRESSION PROFILE ; PANCREATIC-CANCER ; SEROSAL INVASION ; RT-PCR ; CARCINOMA ; SURVIVAL ; IDENTIFICATION ; PROGNOSIS ; SIGNATURE ; TARGETS
摘要	AIM: To detect the expression of 60 microRNAs in gastric cancer tissues and find new predictive biomarkers of gastric cancer with metastasis. METHODS: The expressions of 60 candidate miRNAs in 30 gastric cancer tissues and paired normal tissues were detected by stem-loop real-time reverse transcription-polymerase chain reaction. After primary screening of miRNAs expression, 5 selected miRNAs were further testified in another 22 paired gastric tissues. Based on the expression level of miRNAs and the status of metastasis to lymph node (LN), receiver-operating-characteristic (ROC) curve were used to evaluate their ability in predicting the status of metastasis to LN. RESULTS: Thirty-eight miRNAs expressions in gastric cancer tissues were significantly different from those in paired normal tissues (P < 0.01). Among them, 31 miRNAs were found to be up-expressed in cancer tissues and 1 miRNAs were down-expressed 1.5 fold vs paired normal gastric tissue. Five microRNAs (miR-125a-3p, miR-133b, miR-143, miR-195 and miR-212) were differently expressed between different metastatic groups in 30 gastric cancer biopsies (P < 0.05). Partial correlation analysis showed that hsa-mir-212 and hsamir-195 were correlated with the status of metastasis to LN in spite of age, gender, tumor location, tumor size, depth of invasion and cell differentiation. ROC analysis indicated that miR-212 and miR-195 have better sensitivities (84.6% and 69.2%, respectively) and specificities (both 100%) in distinguishing biopsies with metastasis to LN from biopsies without metastasis to LN. CONCLUSION: miR-212 and miR-195 could be independent biomarkers in predicting the gastric cancer with metastasis to LN. (C) 2011 Baishideng. All rights reserved.
资助项目	Nature Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [Y2080978]; Wenzhou Science and Technology Bureau [H20100028, Y20070067]
出版者	BAISHIDENG PUBLISHING GROUP INC
出版地	PLEASANTON
ISSN	1007-9327
EISSN	2219-2840
卷号	17 期号:31 页码:3645-3651
DOI	10.3748/wjg.v17.i31.3645
页数	7
WOS类目	Gastroenterology & Hepatology
WOS研究方向	Gastroenterology & Hepatology
WOS记录号	WOS:000295062800014
收录类别	SCIE ; PUBMED ; SCOPUS ; CSCD
学科领域	医药、卫生 ; 肿瘤学
URL	查看原文
CSCD记录号	CSCD:4334530
PubMed ID	21987613
PMC记录号	PMC3180023
SCOPUSEID	2-s2.0-80053063873
通讯作者地址	[Shen, Xian]Wenzhou Med Coll, Affiliated Hosp 1, Dept Gen Surg, Wenzhou 325000, Zhejiang, Peoples R China.
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/12181
专题	附属第一医院基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学微生物学
通讯作者	Shen, Xian
作者单位	1.Wenzhou Med Coll, Affiliated Hosp 1, Dept Gen Surg, Wenzhou 325000, Zhejiang, Peoples R China; 2.Wenzhou Med Coll, Dept Microbiol, Wenzhou 325000, Zhejiang, Peoples R China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Wu, Wen-Yi,Xue, Xiang-Yang,Chen, Zhe-Jing,et al. Potentially predictive microRNAs of gastric cancer with metastasis to lymph node[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2011,17(31):3645-3651.
APA	Wu, Wen-Yi., Xue, Xiang-Yang., Chen, Zhe-Jing., Han, Shao-Liang., Huang, Ying-Peng., ... & Shen, Xian. (2011). Potentially predictive microRNAs of gastric cancer with metastasis to lymph node. WORLD JOURNAL OF GASTROENTEROLOGY, 17(31), 3645-3651.
MLA	Wu, Wen-Yi,et al."Potentially predictive microRNAs of gastric cancer with metastasis to lymph node".WORLD JOURNAL OF GASTROENTEROLOGY 17.31(2011):3645-3651.