Application of a liquid chromatography-tandem mass spectrometry method to the pharmacokinetics, bioavailability and tissue distribution of neohesperidin dihydrochalcone in rats

doi:10.3109/00498254.2013.861950

科研成果详情

题名	Application of a liquid chromatography-tandem mass spectrometry method to the pharmacokinetics, bioavailability and tissue distribution of neohesperidin dihydrochalcone in rats
作者	Wang, Xianqin1; Pan, Yu 1; Jianshe, Ma 2; Shi, Shaohua1; Zheng, Xiaoyong 1; Xiang, Zheng 1
发表日期	2014-06
发表期刊	XENOBIOTICA 影响因子和分区
语种	英语
原始文献类型	Article
关键词	LC-MS neohesperidin dihydrochalcone pharmacokinetic tissue distribution
其他关键词	SWEETENERS
摘要	1. This study was aimed at developing a high sensitive and selective liquid chromatography-tandem mass spectrometry method to quantify neohesperidin dihydrochalcone (NHDC) in rat plasma and tissues for pharmacokinetic, bioavailability and tissue distribution studies. 2. Biological samples were processed with one-step protein precipitation. Rutin was chosen as the internal standard (IS). Chromatographical separation was achieved on an SB-C-18 (2.1mm x 150 mm, 5 mu m) column with acetonitrile-0.1% formic acid in water as the mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in negative ion mode; selected ion monitoring mode was used for quantification using target fragment ions m/z 611.4 for NHDC and m/z 609.1 for IS. 3. Calibration plots were linear over the range of 10-3000 ng/mL for NHDC. Lower limit of quantification (LLOQ) for NHDC was 10 ng/mL. Mean recovery of NHDC from plasma and tissues was better than 80.3%. Coefficient of variation of intra-day and inter-day precision were both less than 15%. The bioavailability of NHDC was 21.8%. 4. In conclusion, a sensitive, simple and specific LC-ESI-MS method for the determination of NHDC in rat biological samples was developed. This developed method is successfully used in the pharmacokinetic and tissue distribution study of NHDC in rats.
出版者	INFORMA HEALTHCARE
出版地	LONDON
ISSN	0049-8254
EISSN	1366-5928
卷号	44 期号:6 页码:555-561
DOI	10.3109/00498254.2013.861950
页数	7
WOS类目	Pharmacology & Pharmacy ; Toxicology
WOS研究方向	Pharmacology & Pharmacy ; Toxicology
WOS记录号	WOS:000336058400008
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	24274324
SCOPUSEID	2-s2.0-84900451497
通讯作者地址	[Xiang, Zheng]School of Pharmacy, Wenzhou Medical University,China
Scopus学科分类	Biochemistry;Toxicology;Pharmacology;Health, Toxicology and Mutagenesis
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/12161
专题	药学院（分析测试中心）基础医学院（机能实验教学中心）_基础医学实验教学中心_机能实验教学中心（挂靠、校级）
通讯作者	Xiang, Zheng
作者单位	1.School of Pharmacy, Wenzhou Medical University,China; 2.Function Experiment Teaching Center, Wenzhou Medical University,China
第一作者单位	药学院（分析测试中心）
通讯作者单位	药学院（分析测试中心）
第一作者的第一单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Wang, Xianqin,Pan, Yu,Jianshe, Ma,et al. Application of a liquid chromatography-tandem mass spectrometry method to the pharmacokinetics, bioavailability and tissue distribution of neohesperidin dihydrochalcone in rats[J]. XENOBIOTICA,2014,44(6):555-561.
APA	Wang, Xianqin, Pan, Yu, Jianshe, Ma, Shi, Shaohua, Zheng, Xiaoyong, & Xiang, Zheng. (2014). Application of a liquid chromatography-tandem mass spectrometry method to the pharmacokinetics, bioavailability and tissue distribution of neohesperidin dihydrochalcone in rats. XENOBIOTICA, 44(6), 555-561.
MLA	Wang, Xianqin,et al."Application of a liquid chromatography-tandem mass spectrometry method to the pharmacokinetics, bioavailability and tissue distribution of neohesperidin dihydrochalcone in rats".XENOBIOTICA 44.6(2014):555-561.