Anti-Cancer Effect of alpha-Solanine by Down-Regulating S100P Expression in Colorectal Cancer Cells

doi:10.2174/1574892813666180329163945

科研成果详情

题名	Anti-Cancer Effect of alpha-Solanine by Down-Regulating S100P Expression in Colorectal Cancer Cells
作者	Ni, Xiaofen1; Chen, Jiajun2; Lu, Fengying 3; Yuan, Zhengzhong1; Xu, Xiaofeng1; Hu, Zhen2; Jiang, Lei3
发表日期	2018-12-31
发表期刊	RECENT PATENTS ON ANTI-CANCER DRUG DISCOVERY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Anti-tumor effect apoptosis cell-cycle arrest colorectal cancer invasion migration S100P alpha-solanine
其他关键词	IN-VITRO ; STEROIDAL GLYCOALKALOIDS ; NIGRUM L. ; METASTASIS ; APOPTOSIS ; INVASION ; INHIBITION ; GROWTH ; OVEREXPRESSION ; SOLAMARGINE
摘要	Background: alpha-Solanine, the most important and active component of Solanum nigrum, was found to have anti-cancer activity on multiple cancer cells. However, its effects on colorectal cancer (CRC) and associated molecular mechanisms remain to be further elucidated. Objective: In this study, we investigated the anti-cancer effects of alpha-solanine against CRC cells in vitro and in vivo. Materials & Methods: Cell viability was measured using Cell Counting Kit-8 (CCK-8) assay; cell cycle was analyzed with a Cycletest Plus DNA Reagent Kit; cell apoptosis was detected by flow cytometer; cell migration and invasive ability was determined by Transwell assays; S100P protein expression was also analyzed by western blotting; lentiviral vectors expressing shRNA targeting the S100P gene. Results: We demonstrated that alpha-solanine inhibited CRC cell (SW480, SW620 and HT-29) growth as well as migration and invasion, induced cell cycle arrest and apoptosis in vitro, and suppressed tumor growth in vivo. Moreover, we observed that S100P expression was downregulated by alpha-solanine. Overexpression of S100P partially reversed the a-solanine-induced growth inhibition of CRC cells. Conversely, knockdown of S100P by lentiviral-mediated RNAi resulted in significantly promoting the a-solanine-induced growth inhibition. Conclusion: These findings suggest that alpha-solanine is a potential agent for the treatment of CRC, and the anti-tumor effect of alpha-solanine in the CRC cells may be mediated at least partly by the downregulation of S100P.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81672385]; Wenzhou Science & Technological Project [Y20140484]
出版者	BENTHAM SCIENCE PUBL LTD
出版地	SHARJAH
ISSN	1574-8928
EISSN	2212-3970
卷号	13 期号:2 页码:240-247
DOI	10.2174/1574892813666180329163945
页数	8
WOS类目	Oncology ; Pharmacology & Pharmacy
WOS研究方向	Oncology ; Pharmacology & Pharmacy
WOS记录号	WOS:000432224500008
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	29600769
SCOPUSEID	2-s2.0-85049171804
通讯作者地址	[Jiang, Lei]Central Laboratory,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Oncology;Drug Discovery;Cancer Research;Pharmacology (medical)
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/12120
专题	附属第一医院_中医科附属第二医院第二临床医学院、附属第二医院、育英儿童医院第一临床医学院（信息与工程学院）、附属第一医院_中心实验室
通讯作者	Jiang, Lei
作者单位	1.Department of Traditional Chinese Medicine,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Department of Traditional Chinese Medicine,The Second Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 3.Central Laboratory,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Ni, Xiaofen,Chen, Jiajun,Lu, Fengying,et al. Anti-Cancer Effect of alpha-Solanine by Down-Regulating S100P Expression in Colorectal Cancer Cells[J]. RECENT PATENTS ON ANTI-CANCER DRUG DISCOVERY,2018,13(2):240-247.
APA	Ni, Xiaofen., Chen, Jiajun., Lu, Fengying., Yuan, Zhengzhong., Xu, Xiaofeng., ... & Jiang, Lei. (2018). Anti-Cancer Effect of alpha-Solanine by Down-Regulating S100P Expression in Colorectal Cancer Cells. RECENT PATENTS ON ANTI-CANCER DRUG DISCOVERY, 13(2), 240-247.
MLA	Ni, Xiaofen,et al."Anti-Cancer Effect of alpha-Solanine by Down-Regulating S100P Expression in Colorectal Cancer Cells".RECENT PATENTS ON ANTI-CANCER DRUG DISCOVERY 13.2(2018):240-247.