大鼠心肌组织提取物诱导骨髓基质干细胞分化为心肌样细胞

科研成果详情

题名	大鼠心肌组织提取物诱导骨髓基质干细胞分化为心肌样细胞
其他题名	Evidence of Tissue Extracts from Infarcted Myocardium of rat in Promoting the Differentiation of Bone Marrow Stromal Cells into Cardiomyocyte-like Cells
作者	尹琪
学位类型	硕士
导师	孙成超
答辩日期	2009-12-14
学位授予单位	温州医科大学
学位专业	外科学
关键词	骨髓基质干细胞细胞分化心脏组织提取液心肌梗死
摘要	目的: 化学方法和共培养技术已经被证明可以诱导骨髓基质干细胞分化为心肌细胞.我们设想大鼠的心肌组织提取物是否可以模拟心脏微环境同时做为一种自然诱导剂以诱导干细胞分化为心肌细胞.方法: 本实验分4个组: 正常对照组、乳鼠心肌细胞裂解液组、正常心肌组织提取物组、梗死心肌组织提取物组.经过1、3、5、7天的诱导后进行RT-PCR、免疫细胞化学以及电镜检测.结果: 经过7天的诱导,细胞体积变大,外观呈多角形.电镜检测发现明显的肌丝、条纹状的肌节以及增多的线粒体.RT-PCR和免疫细胞化学发现心肌特异性的转录因子和蛋白的表达.并且, 梗死心肌组织提取物组较其他2组有更强的促分化能力.结论:我们的研究结果显示心肌组织提取物特别是梗死心肌组织提取物能有效的促进骨髓基质干细胞分化为心肌样细胞.
其他摘要	Objective Chemical induction and Co-culture techniques had been indicated to promote the differentiation of bone marrow stromal cells (BMSCs) into cardiomyocytes. We investigate whether cardiac tissue extracts from rat could mimic the cardiac microenvironment and act as a natural inducer in promoting the differentiation of BMSCs towards cardiomyocytes. Methods In this study, we employed three kinds of tissue extracts or cell lysate, i.e. infracted myocardial tissue extracts (IMTE), normal myocardial tissue extracts (NMTE) and cultured neonatal myocardial lysate (NML) to induce BMSCs into cardiomyocyte-like cell. The cells were harvested at each time point for RT-PCR detection, immunocytochemical analyses, and transmission electron microscopy. Results After a 7-day induction BMSCs shaped to be enlarged and polygonal in morphology. Myofilaments, striated sarcomeres, Z-lines, and more mitochondia were observed by transmission electron microscopy, and elevated expression levels of cardiac-specific genes and proteins were also confirmed by reverse transcription-polymerase chain reaction and immunocytochemistry. Moreover, IMTE showed a greater capacity in differentiating BMSCs into cardiomyocyte-like cells. Conclusions Our study demonstrates that cardiac tissue extracts, especially IMTE, could differentiate BMSCs into cardiomycyte-like cells more effectively
语种	中文
学号	20051344
发布年限	2010-05-27
毕业论文分类号	0R05
原始专题	第一临床学院
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文献类型	学位论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/120196
专题	温州医科大学
作者单位	溫州医科大学第一临床学院
推荐引用方式 GB/T 7714	尹琪. 大鼠心肌组织提取物诱导骨髓基质干细胞分化为心肌样细胞[D]. 温州医科大学,2009.