壳聚糖胶原复合物在兔角膜基质层间的组织相容性研究

科研成果详情

题名	壳聚糖胶原复合物在兔角膜基质层间的组织相容性研究
其他题名	Study on Histocompatibility of Rabbit Cornea with Complexes of Collage- Chitosan membrane
作者	周晓伟
学位类型	硕士
导师	吕帆
答辩日期	2010-05-18
学位授予单位	温州医科大学
学位专业	眼科学
关键词	壳聚糖膜，壳聚糖胶原复合膜，组织相容性
摘要	目的探讨壳聚糖胶原复合膜(collagen-chitosan)植入兔角膜基质层间的组织相容性，以评价其作为人组织工程角膜修复支架材料的可行性，为角膜损伤修复寻找更加合适的角膜支架材料。方法选用20只新西兰大白兔，在其角膜基质层间约2/3深度处分离出板层囊袋，将制作好的大小约4 mm× 4 mm，厚度约20μm的壳聚糖膜，壳聚糖胶原复合膜片植入囊袋内，根据植入膜片材料的不同分为A、B三组，A组（10只）植入壳聚糖胶原复合膜，B组（10只）植入壳聚糖膜以作对照。术后第1天、第4天、第1周，及以后每周的相同时间点都由同一观察者，通过裂隙灯显微镜检查术眼角膜情况并记录术眼球结膜充血、角膜水肿、前房闪辉、角膜新生血管以及材料的变化等情况，并于术后第8周取术眼角膜组织制作病理学切片进行HE染色观察，比较观察植入材料对角膜组织的不同影响，以对壳聚糖胶原复合膜的组织相容性作出初步的评价。结果术后在观察时间8周内，壳聚糖以及壳聚糖胶原复合膜片在兔角膜基质层间在位、无脱出；术后各观察时间点，A组壳聚糖胶原复合膜组动物术眼炎症反应轻于B组壳聚糖膜组，且未见角膜新生血管发生，B组共5例术眼出现角膜新生血管，但范围未超过1∕3角膜直径，局限于一个象限内，且未接近植片周围，观察时间内尚未消退，两组新生血管长入差异有显著统计学差异（P<0.05）；A、B组膜片均于术后第2周开始从周边部出现降解，在相同观察时间点，A组壳聚糖胶原复合膜降解程度临床观察略小于B组壳聚糖膜，但并无统计学差异（P>0.05）。HE染色显示A组壳聚糖胶原复合膜膜片周围炎性细胞浸润轻于B组壳聚糖膜组，降解产物与角膜基质纤维融合。结论壳聚糖胶原复合膜与活体兔角膜基质组织相容性比壳聚糖膜更优，更少引发炎症反应及角膜新生血管的发生，其用作角膜损伤修复材料具有广阔前景，但是植入材料的长期安全稳定性及降解性能、降解产物的去向及对角膜组织的影响需要进一步的研究。
其他摘要	Objective To study the histocompatibility of collagen-chitosan membrane after been implanted into the corneal stroma of rabbits so as to evaluate its feasibility of being used as cornea repair materials. Methods 20 New-zealand rabbits were used and equally separated into Group A and Group B according to different implanted membranes, Group A with Collagen-chitosan membrane while Group B with chitosan membrane. Conjunctival congestion, corneal edema, aqueous flare, corneal neovascularization and the changes of the materials were observed and recorded clinically postoperation on the scheduled time point, and the corneas implanted with biomaterial membranes were taken for histological examination at the 8th week after operations. Results No obvious immunological rejection was observed in corneas of Group A during the experimental periods. The inﬂammatory reaction of corneas in Group A was less than that in Group B. There were visible neovessels in Group B from 6Pth Pweek, yet there was none in Group A and significant difference was found between them during the observation period(P<0.05).The degradation of biomaterials began at the 2Pth Pweek after implantation in both groups but the rate of degradation of Group A was slower than that of Group B at the same observation point yet there was no significant difference between them(P>0.05).According to the histological observation, the degree of the inﬂammatory reaction in surrounding corneal stroma ,Group A was less than that in Group B observed at the 8th week postoperatively. The corneal collagen fibers were mixed with the patches of the degradation products of membranes in both groups. Conclusions Collagen-chitosan polymers presented better histocmpatibility with rabbits corneal stoma than Chitosan and would potentially be used as the scaffold of tissue-engineered cornea, but the long-term observation of the biomaterials implanted into the cornea need further reaserch.
语种	中文
学号	20071360
发布年限	2010-05-27
毕业论文分类号	0R77
原始专题	眼视光学院
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全文文件名	20071360周晓伟2010眼科学.pdf\|20071360周晓伟2010眼科学.pdf
文献类型	学位论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/119365
专题	温州医科大学
作者单位	溫州医科大学眼视光学院
第一作者单位	温州医科大学
推荐引用方式 GB/T 7714	周晓伟. 壳聚糖胶原复合物在兔角膜基质层间的组织相容性研究[D]. 温州医科大学,2010.