Metabolic alterations in the rat cerebellum following acute middle cerebral artery occlusion, as determined by H-1 NMR spectroscopy

doi:10.3892/mmr.2017.7918

科研成果详情

题名	Metabolic alterations in the rat cerebellum following acute middle cerebral artery occlusion, as determined by H-1 NMR spectroscopy
作者	Hu, Zi-Long 1; Xia, Huan-Huan 2,3; Yang, Yun-Jun1; Zheng, Hong2; Zhao, Liang-Cai2; Chen, Yong-Chun1; Zhuge, Qi-Chuan1; Xia, Neng-Zhi1; Gao, Hong-Chang2; Chen, Wei-Jian1
发表日期	2018-01
发表期刊	MOLECULAR MEDICINE REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	middle cerebral artery occlusion ischemia rat cerebellum metabolites H-1 NMR
其他关键词	MAGNETIC-RESONANCE-SPECTROSCOPY ; ACUTE ISCHEMIC-STROKE ; POSITRON-EMISSION-TOMOGRAPHY ; BLOOD-FLOW ; HUMAN BRAIN ; NEUROCHEMICAL PROFILE ; NEUROLOGICAL DEFICIT ; WEIGHTED MRI ; EX-VIVO ; DIASCHISIS
摘要	Supratentorial focal ischemia may reduce cerebral blood volume and cerebellar glucose metabolic rate contralateral to the region of ischemia. The present study investigated the effects of middle cerebral artery occlusion (MCAO) on cerebral metabolism in the ischemic cerebral hemisphere and the non-ischemic cerebellum in rats 1, 3, 9 and 24 h following ischemia using ex vivo proton nuclear magnetic resonance (H-1 NMR) spectroscopy. The results demonstrated that focal ischemia induced increases in the levels of lactate and alanine, and a decrease in succinate, as early as 1 h following ischemia in the left cerebral hemisphere and the right cerebellum. A continuous increase in lactate levels and decrease in creatine levels were detected in both cerebral areas 3 and 24 h post-MCAO. The most obvious difference between the two cerebral areas was that there was no statistically significant difference in N-acetyl aspartate (NAA) levels in the right cerebellum at all time points; however, the amino acid levels of NAA in the left cerebral hemisphere were markedly decreased 3, 9 and 24 h post-MCAO. In addition, an obvious increase in glutamine was observed in the right and left cerebellum at 3, 9 and 24 h post-MCAO. Furthermore, the present study demonstrated that -aminobutyric acid levels were decreased at 1 h in the left and right cerebellum and were evidently increased at 24 h in the right cerebellum post-MCAO. In conclusion, supratentorial ischemia has been indicated to affect the activities of the non-ischemic contralateral cerebellum. Therefore, these results suggested that an NMR-based metabonomic approach may be used as a potential means to elucidate cerebral and cerebellar metabolism following MCAO, which may help improve understanding regarding cerebral infarction at a molecular level. Ex vivo(1)H NMR analysis may be useful for the assessment of clinical biopsies.
资助项目	Fund of Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research [2012E10008, LH001]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81571626, 21575105]; Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [LY15H220001]; Medical Science and Technology Project of Zhejiang Province [2014KYA134]; Science and Technology Planning Project of Wenzhou City [Y20140731]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1791-2997
EISSN	1791-3004
卷号	17 期号:1 页码:531-541
DOI	10.3892/mmr.2017.7918
页数	11
WOS类目	Oncology ; Medicine, Research & Experimental
WOS研究方向	Oncology ; Research & Experimental Medicine
WOS记录号	WOS:000423433300069
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	29115616
SCOPUSEID	2-s2.0-85034970240
通讯作者地址	[Chen, Wei-Jian]Department of Radiology,First Affiliated Hospital,Wenzhou Medical University,Zhejiang Province Key Laboratory of Aging and Neurological Disorder Research,2 Fuxue Lane,Wenzhou, Zhejiang,325000,China
Scopus学科分类	Biochemistry;Molecular Medicine;Molecular Biology;Genetics;Oncology;Cancer Research
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/11935
专题	第一临床医学院（信息与工程学院）、附属第一医院_影像医学与核医学_放射科药学院（分析测试中心）附属第一医院附属第二医院_核磁共振室其他_附属第三医院（瑞安市人民医院）
通讯作者	Chen, Wei-Jian
作者单位	1.Department of Radiology,First Affiliated Hospital,Wenzhou Medical University,Zhejiang Province Key Laboratory of Aging and Neurological Disorder Research,2 Fuxue Lane,Wenzhou, Zhejiang,325000,China; 2.Institute of Metabonomics and Medical NMR,School of Pharmaceutical Sciences,Wenzhou Medical University,University Town,106 Nanliu Road,Wenzhou, Zhejiang,325000,China; 3.Department of Pharmacy,Third Affiliated Hospital,Wenzhou Medical University,Rui'an, Zhejiang,325200,China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Hu, Zi-Long,Xia, Huan-Huan,Yang, Yun-Jun,et al. Metabolic alterations in the rat cerebellum following acute middle cerebral artery occlusion, as determined by H-1 NMR spectroscopy[J]. MOLECULAR MEDICINE REPORTS,2018,17(1):531-541.
APA	Hu, Zi-Long., Xia, Huan-Huan., Yang, Yun-Jun., Zheng, Hong., Zhao, Liang-Cai., ... & Chen, Wei-Jian. (2018). Metabolic alterations in the rat cerebellum following acute middle cerebral artery occlusion, as determined by H-1 NMR spectroscopy. MOLECULAR MEDICINE REPORTS, 17(1), 531-541.
MLA	Hu, Zi-Long,et al."Metabolic alterations in the rat cerebellum following acute middle cerebral artery occlusion, as determined by H-1 NMR spectroscopy".MOLECULAR MEDICINE REPORTS 17.1(2018):531-541.