Autophagy regulates the therapeutic potential of adipose-derived stem cells in LPS-induced pulmonary microvascular barrier damage

doi:10.1038/s41419-019-2037-8

科研成果详情

题名	Autophagy regulates the therapeutic potential of adipose-derived stem cells in LPS-induced pulmonary microvascular barrier damage
作者	Li, Chichi1; Pan, Jingye2; Ye, Lechi3; Xu, Honglei4; Wang, Beibei 5; Xu, Hanyan5; Xu, Lingna 5; Hou, Tongtong 5; Zhang, Dan5
发表日期	2019-10-23
发表期刊	CELL DEATH & DISEASE 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	INDUCED LUNG INJURY ; ENDOTHELIAL BARRIER ; GROWTH-FACTOR ; LIPOPOLYSACCHARIDE ; METABOLISM ; MAINTAINS ; APOPTOSIS ; PROMOTES ; SURVIVAL ; MODEL
摘要	Adipose-derived stem cells (ADSCs) have been shown to be beneficial in some pulmonary diseases, and the paracrine effect is the major mechanism underlying ADSC-based therapy. Autophagy plays a crucial role in maintaining stem cell homeostasis and survival. However, the role of autophagy in mediating ADSC paracrine effects has not been thoroughly elucidated. We examined whether ADSCs participate in lipopolysaccharide (LPS)-induced pulmonary microvascular endothelial cell (PMVEC) barrier damage in a paracrine manner and illuminated the role of autophagy in regulating ADSC paracrine effects. PMVECs and ADSCs with or without autophagy inhibition were cocultured without intercellular contact, and the microvascular barrier function was assessed after LPS treatment. ADSC paracrine function was evaluated by detecting essential growth factors for endothelial cells. For in vivo experiments, ADSCs with or without autophagy inhibition were transplanted into LPS-induced lung-injury mice, and lung injury was assessed. ADSCs significantly alleviated LPS-induced microvascular barrier injury. In addition, ADSC paracrine levels of VEGF, FGF, and EGF were induced by LPS treatment, especially in the coculture condition. Inhibiting autophagy weakened the paracrine function and the protective effects of ADSCs on microvascular barrier injury. Moreover, ADSC transplantation alleviated LPS-induced lung injury, and inhibiting autophagy markedly weakened the therapeutic effect of ADSCs on lung injury. Together, these findings show that ADSC paracrine effects play a vital protective role in LPS-induced pulmonary microvascular barrier injury. Autophagy is a positive mediating factor in the paracrine process. These results are helpful for illuminating the role and mechanism of ADSC paracrine effects and developing effective therapies in acute lung injury.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81800075]; Wenzhou Science and Technology Project [Y20190116]
出版者	NATURE PUBLISHING GROUP
出版地	LONDON
ISSN	2041-4889
卷号	10 期号:11 页码:804
DOI	10.1038/s41419-019-2037-8
页数	15
WOS类目	Cell Biology
WOS研究方向	Cell Biology
WOS记录号	WOS:000493383500002
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	31645547
PMC记录号	PMC6811543
SCOPUSEID	2-s2.0-85074087250
通讯作者地址	[Zhang, Dan]Department of Respiratory Medicine,The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang,Wenzhou City,325000,China
Scopus学科分类	Immunology;Cellular and Molecular Neuroscience;Cell Biology;Cancer Research
TOP期刊	TOP期刊
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/11710
专题	第一临床医学院（信息与工程学院）、附属第一医院_外科学_整形外科附属第一医院附属第一医院_重症医学科（ICU）
通讯作者	Zhang, Dan
作者单位	1.Plastic Surgery,The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang,Wenzhou City,325000,China; 2.Department of Intensive Care Unit,The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang,Wenzhou City,325000,China; 3.Department of Colorectal & Anal Surgery,The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang,Wenzhou City,325000,China; 4.Emergency Department,The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang,Wenzhou City,325000,China; 5.Department of Respiratory Medicine,The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang,Wenzhou City,325000,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Li, Chichi,Pan, Jingye,Ye, Lechi,et al. Autophagy regulates the therapeutic potential of adipose-derived stem cells in LPS-induced pulmonary microvascular barrier damage[J]. CELL DEATH & DISEASE,2019,10(11):804.
APA	Li, Chichi., Pan, Jingye., Ye, Lechi., Xu, Honglei., Wang, Beibei., ... & Zhang, Dan. (2019). Autophagy regulates the therapeutic potential of adipose-derived stem cells in LPS-induced pulmonary microvascular barrier damage. CELL DEATH & DISEASE, 10(11), 804.
MLA	Li, Chichi,et al."Autophagy regulates the therapeutic potential of adipose-derived stem cells in LPS-induced pulmonary microvascular barrier damage".CELL DEATH & DISEASE 10.11(2019):804.