题名 | Autophagy regulates the therapeutic potential of adipose-derived stem cells in LPS-induced pulmonary microvascular barrier damage |
作者 | |
发表日期 | 2019-10-23 |
发表期刊 | CELL DEATH & DISEASE 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article |
其他关键词 | INDUCED LUNG INJURY ; ENDOTHELIAL BARRIER ; GROWTH-FACTOR ; LIPOPOLYSACCHARIDE ; METABOLISM ; MAINTAINS ; APOPTOSIS ; PROMOTES ; SURVIVAL ; MODEL |
摘要 | Adipose-derived stem cells (ADSCs) have been shown to be beneficial in some pulmonary diseases, and the paracrine effect is the major mechanism underlying ADSC-based therapy. Autophagy plays a crucial role in maintaining stem cell homeostasis and survival. However, the role of autophagy in mediating ADSC paracrine effects has not been thoroughly elucidated. We examined whether ADSCs participate in lipopolysaccharide (LPS)-induced pulmonary microvascular endothelial cell (PMVEC) barrier damage in a paracrine manner and illuminated the role of autophagy in regulating ADSC paracrine effects. PMVECs and ADSCs with or without autophagy inhibition were cocultured without intercellular contact, and the microvascular barrier function was assessed after LPS treatment. ADSC paracrine function was evaluated by detecting essential growth factors for endothelial cells. For in vivo experiments, ADSCs with or without autophagy inhibition were transplanted into LPS-induced lung-injury mice, and lung injury was assessed. ADSCs significantly alleviated LPS-induced microvascular barrier injury. In addition, ADSC paracrine levels of VEGF, FGF, and EGF were induced by LPS treatment, especially in the coculture condition. Inhibiting autophagy weakened the paracrine function and the protective effects of ADSCs on microvascular barrier injury. Moreover, ADSC transplantation alleviated LPS-induced lung injury, and inhibiting autophagy markedly weakened the therapeutic effect of ADSCs on lung injury. Together, these findings show that ADSC paracrine effects play a vital protective role in LPS-induced pulmonary microvascular barrier injury. Autophagy is a positive mediating factor in the paracrine process. These results are helpful for illuminating the role and mechanism of ADSC paracrine effects and developing effective therapies in acute lung injury. |
资助项目 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81800075]; Wenzhou Science and Technology Project [Y20190116] |
出版者 | NATURE PUBLISHING GROUP |
出版地 | LONDON |
ISSN | 2041-4889 |
卷号 | 10期号:11页码:804 |
DOI | 10.1038/s41419-019-2037-8 |
页数 | 15 |
WOS类目 | Cell Biology |
WOS研究方向 | Cell Biology |
WOS记录号 | WOS:000493383500002 |
收录类别 | SCIE ; PUBMED ; SCOPUS |
URL | 查看原文 |
PubMed ID | 31645547 |
PMC记录号 | PMC6811543 |
SCOPUSEID | 2-s2.0-85074087250 |
通讯作者地址 | [Zhang, Dan]Department of Respiratory Medicine,The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang,Wenzhou City,325000,China |
Scopus学科分类 | Immunology;Cellular and Molecular Neuroscience;Cell Biology;Cancer Research |
TOP期刊 | TOP期刊 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/11710 |
专题 | 第一临床医学院(信息与工程学院)、附属第一医院_外科学_整形外科 附属第一医院 附属第一医院_重症医学科(ICU) |
通讯作者 | Zhang, Dan |
作者单位 | 1.Plastic Surgery,The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang,Wenzhou City,325000,China; 2.Department of Intensive Care Unit,The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang,Wenzhou City,325000,China; 3.Department of Colorectal & Anal Surgery,The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang,Wenzhou City,325000,China; 4.Emergency Department,The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang,Wenzhou City,325000,China; 5.Department of Respiratory Medicine,The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang,Wenzhou City,325000,China |
第一作者单位 | 附属第一医院; 第一临床医学院(信息与工程学院)、附属第一医院 |
通讯作者单位 | 附属第一医院; 第一临床医学院(信息与工程学院)、附属第一医院 |
第一作者的第一单位 | 附属第一医院 |
推荐引用方式 GB/T 7714 | Li, Chichi,Pan, Jingye,Ye, Lechi,et al. Autophagy regulates the therapeutic potential of adipose-derived stem cells in LPS-induced pulmonary microvascular barrier damage[J]. CELL DEATH & DISEASE,2019,10(11):804. |
APA | Li, Chichi., Pan, Jingye., Ye, Lechi., Xu, Honglei., Wang, Beibei., ... & Zhang, Dan. (2019). Autophagy regulates the therapeutic potential of adipose-derived stem cells in LPS-induced pulmonary microvascular barrier damage. CELL DEATH & DISEASE, 10(11), 804. |
MLA | Li, Chichi,et al."Autophagy regulates the therapeutic potential of adipose-derived stem cells in LPS-induced pulmonary microvascular barrier damage".CELL DEATH & DISEASE 10.11(2019):804. |
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