In Vivo Cardiotoxicity Induced by Sodium Aescinate in Zebrafish Larvae

doi:10.3390/molecules21030190

科研成果详情

题名	In Vivo Cardiotoxicity Induced by Sodium Aescinate in Zebrafish Larvae
作者	Liang, Jinfeng 1; Jin, Wangdong 2; Li, Hongwen 3; Liu, Hongcui 4; Huang, Yanfeng 4; Shan, Xiaowen 1; Li, Chunqi4,5; Shan, Letian 2; Efferth, Thomas 6
发表日期	2016-03
发表期刊	MOLECULES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	sodium aescinate zebrafish larvae cardiotoxicity MNLC LC10
其他关键词	MODEL ; TOXICITY ; TOXICOLOGY ; CARDIOMYOPATHY ; ANTIOXIDANT ; PROTECTION ; VERTEBRATE ; MUTATION ; EXTRACT ; SCREENS
摘要	Sodium aescinate (SA) is a widely-applied triterpene saponin product derived from horse chestnut seeds, possessing vasoactive and organ-protective activities with oral or injection administration in the clinic. To date, no toxicity or adverse events in SA have been reported, by using routine models (in vivo or in vitro), which are insufficient to predict all aspects of its pharmacological and toxicological actions. In this study, taking advantage of transparent zebrafish larvae (Danio rerio), we evaluated cardiovascular toxicity of SA at doses of 1/10 MNLC, 1/3 MNLC, MNLC and LC10 by yolk sac microinjection. The qualitative and quantitative cardiotoxicity in zebrafish was assessed at 48 h post-SA treatment, using specific phenotypic endpoints: heart rate, heart rhythm, heart malformation, pericardial edema, circulation abnormalities, thrombosis and hemorrhage. The results showed that SA at 1/10 MNLC and above doses could induce obvious cardiac and pericardial malformations, whilst 1/3 MNLC and above doses could induce significant cardiac malfunctions (heart rate and circulation decrease/absence), as compared to untreated or vehicle-treated control groups. Such cardiotoxic manifestations occurred in more than 50% to 100% of all zebrafish treated with SA at MNLC and LC10. Our findings have uncovered the potential cardiotoxicity of SA for the first time, suggesting more attention to the risk of its clinical application. Such a time- and cost-saving zebrafish cardiotoxicity assay is very valid and reliable for rapid prediction of compound toxicity during drug research and development.
资助项目	Science and Technology Project Grant of Zhejiang Food and Drug Administration system [2014002]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81302989]; Zhejiang Provincial Science and Technology Project of Traditional Chinese Medicine of China [2013ZQ007]; Independent Project of Zhejiang Provincial Scientific and Technological Innovation Team of China [2011R50022-02]; Research Fund for the Doctoral Program of Higher Education of ChinaResearch Fund for the Doctoral Program of Higher Education of China (RFDP)Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) [20133322120006]; Scientific Research Project of the Zhejiang Provincial Department of Education [201226186]; Ningbo Natural Science Foundation [2014A610277]
出版者	MDPI AG
出版地	BASEL
ISSN	1420-3049
卷号	21 期号:3
DOI	10.3390/molecules21030190
页数	10
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:000373802200133
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	26907249
SCOPUSEID	2-s2.0-84963713737
通讯作者地址	[Liang, Jinfeng]Zhejiang Center for Drugs and Cosmetics Evaluation,Zhejiang Province Food and Drug Administration,Hangzhou,310012,China
Scopus学科分类	Analytical Chemistry;Chemistry (miscellaneous);Molecular Medicine;Pharmaceutical Science;Drug Discovery;Physical and Theoretical Chemistry;Organic Chemistry
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/10840
专题	检验医学院（生命科学学院、生物学实验教学中心）_模式生物技术与应用重点实验室
通讯作者	Liang, Jinfeng
作者单位	1.Zhejiang Center for Drugs and Cosmetics Evaluation,Zhejiang Province Food and Drug Administration,Hangzhou,310012,China; 2.Institute of Orthopaedics and Traumatology,Zhejiang Chinese Medical University,No. 548 Binwen Road,Binjiang District, Hangzhou,310053,China; 3.Experimental and Training Center,Zhejiang Pharmaceutical College,Ningbo,315100,China; 4.Hunter Biotechnology,Inc.,Transfarland, Hangzhou,310012,China; 5.Zhejiang Provincial Key Lab for Technology and Application of Model Organisms,Wenzhou Medical University,Wenzhou,325035,China; 6.Department of Pharmaceutical Biology,Institute of Pharmacy and Biochemistry,Johannes Gutenberg University,Mainz,55128,Germany
推荐引用方式 GB/T 7714	Liang, Jinfeng,Jin, Wangdong,Li, Hongwen,et al. In Vivo Cardiotoxicity Induced by Sodium Aescinate in Zebrafish Larvae[J]. MOLECULES,2016,21(3).
APA	Liang, Jinfeng., Jin, Wangdong., Li, Hongwen., Liu, Hongcui., Huang, Yanfeng., ... & Efferth, Thomas. (2016). In Vivo Cardiotoxicity Induced by Sodium Aescinate in Zebrafish Larvae. MOLECULES, 21(3).
MLA	Liang, Jinfeng,et al."In Vivo Cardiotoxicity Induced by Sodium Aescinate in Zebrafish Larvae".MOLECULES 21.3(2016).