The full transcription map of mouse papillomavirus type 1 (MmuPV1) in mouse wart tissues

doi:10.1371/journal.ppat.1006715

科研成果详情

题名	The full transcription map of mouse papillomavirus type 1 (MmuPV1) in mouse wart tissues
作者	Xue, Xiang-Yang1,2; Majerciak, Vladimir 1; Uberoi, Aayushi 3; Kim, Bong-Hyun 4,5; Gotte, Deanna 1; Chen, Xiongfong 4,5; Cam, Maggie 4; Lambert, Paul F.3; Zheng, Zhi-Ming 1
发表日期	2017-11
发表期刊	PLOS PATHOGENS 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	IN-SITU HYBRIDIZATION ; LATE GENE-EXPRESSION ; VIRAL REPLICATION ; MESSENGER-RNAS ; CODON USAGE ; LIFE-CYCLE ; CELL-LINES ; PROTEIN ; DIFFERENTIATION ; DNA
摘要	Mouse papillomavirus type 1 (MmuPV1) provides, for the first time, the opportunity to study infection and pathogenesis of papillomaviruses in the context of laboratory mice. In this report, we define the transcriptome of MmuPV1 genome present in papillomas arising in experimentally infected mice using a combination of RNA-seq, PacBio Iso-seq, 5' RACE, 3' RACE, primer-walking RT-PCR, RNase protection, Northern blot and in situ hybridization analyses. We demonstrate that the MmuPV1 genome is transcribed unidirectionally from five major promoters (P) or transcription start sites (TSS) and polyadenylates its transcripts at two major polyadenylation (pA) sites. We designate the P-7503, P-360 and P-859 as early promoters because they give rise to transcripts mostly utilizing the polyadenylation signal at nt 3844 and therefore can only encode early genes, and P-7107 and P-533 as late promoters because they give rise to transcripts utilizing polyadenylation signals at either nt 3844 or nt 7047, the latter being able to encode late, capsid proteins. MmuPV1 genome contains five splice donor sites and three acceptor sites that produce thirty-six RNA isoforms deduced to express seven predicted early gene products (E6, E7, E1, E1(boolean AND)M1, E1(boolean AND)M2, E2 and E8(boolean AND)E2) and three predicted late gene products (E1(boolean AND)E4, L2 and L1). The majority of the viral early transcripts are spliced once from nt 757 to 3139, while viral late transcripts, which are predicted to encode L1, are spliced twice, first from nt 7243 to either nt 3139 (P7107) or nt 757 to 3139 (P533) and second from nt 3431 to nt 5372. Thirteen of these viral transcripts were detectable by Northern blot analysis, with the P533-derived late E1(boolean AND)E4 transcripts being the most abundant. The late transcripts could be detected in highly differentiated keratinocytes of MmuPV1-infected tissues as early as ten days after MmuPV1 inoculation and correlated with detection of L1 protein and viral DNA amplification. In mature warts, detection of L1 was also found in more poorly differentiated cells, as previously reported. Subclinical infections were also observed. The comprehensive transcription map of MmuPV1 generated in this study provides further evidence that MmuPV1 is similar to high-risk cutaneous beta human papillomaviruses. The knowledge revealed will facilitate the use of MmuPV1 as an animal virus model for understanding of human papillomavirus gene expression, pathogenesis and immunology.
资助项目	intramural program of National Institutes of Health, National Cancer Institute, Center for Cancer Research [NCI ZIASC010357]; NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [CA022443, CA210807, ARO 66524]; NATIONAL CANCER INSTITUTEUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Cancer Institute (NCI) [R35CA210807, ZIASC010357, P01CA022443] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) [P30AR066524] Funding Source: NIH RePORTER
出版者	PUBLIC LIBRARY SCIENCE
出版地	SAN FRANCISCO
ISSN	1553-7366
EISSN	1553-7374
卷号	13 期号:11 页码:e1006715.
DOI	10.1371/journal.ppat.1006715
页数	36
WOS类目	Microbiology ; Parasitology ; Virology
WOS研究方向	Microbiology ; Parasitology ; Virology
WOS记录号	WOS:000416888500025
收录类别	SCIE ; PUBMED
URL	查看原文
Pubmed记录号	29176795
PMC记录号	PMC5720830
通讯作者地址	[Zheng, Zhi-Ming]NCI, Tumor Virus RNA Biol Sect, RNA Biol Lab, Ctr Canc Res,NIH, Frederick, MD 21701 USA.
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/10837
专题	检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系
通讯作者	Zheng, Zhi-Ming
作者单位	1.NCI, Tumor Virus RNA Biol Sect, RNA Biol Lab, Ctr Canc Res,NIH, Frederick, MD 21701 USA; 2.Wenzhou Med Univ, Dept Microbiol & Immunol, Wenzhou, Zhejiang, Peoples R China; 3.Univ Wisconsin, Sch Med & Publ Hlth, McArdle Lab Canc Res, Madison, WI USA; 4.NCI, Collaborat Bioinformat Resource, Ctr Canc Res, NIH, Bethesda, MD 20892 USA; 5.Frederick Natl Lab Canc Res, Leidos Biomed Res, Frederick, MD USA
第一作者单位	温州医科大学
推荐引用方式 GB/T 7714	Xue, Xiang-Yang,Majerciak, Vladimir,Uberoi, Aayushi,et al. The full transcription map of mouse papillomavirus type 1 (MmuPV1) in mouse wart tissues[J]. PLOS PATHOGENS,2017,13(11):e1006715..
APA	Xue, Xiang-Yang., Majerciak, Vladimir., Uberoi, Aayushi., Kim, Bong-Hyun., Gotte, Deanna., ... & Zheng, Zhi-Ming. (2017). The full transcription map of mouse papillomavirus type 1 (MmuPV1) in mouse wart tissues. PLOS PATHOGENS, 13(11), e1006715..
MLA	Xue, Xiang-Yang,et al."The full transcription map of mouse papillomavirus type 1 (MmuPV1) in mouse wart tissues".PLOS PATHOGENS 13.11(2017):e1006715..