Major Immunodominant Region of Hepatitis B Virus Core Antigen as a Delivery Vector to Improve the Immunogenicity of the Fusion Antigen ROP2-SAG1 Multiepitope from Toxoplasma gondii in Mice

doi:10.1089/vim.2016.0135

科研成果详情

题名	Major Immunodominant Region of Hepatitis B Virus Core Antigen as a Delivery Vector to Improve the Immunogenicity of the Fusion Antigen ROP2-SAG1 Multiepitope from Toxoplasma gondii in Mice
作者	Wang, Wenhuan; Feng, Fangfang; Lv, Jinhui; Xie, Zixin; Chen, Jun; Zhang, Lifang; Li, Wenshu
发表日期	2017-09
发表期刊	VIRAL IMMUNOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Toxoplasma dominant multiepitope hepatitis B core antigen vector immune response
其他关键词	ELECTRON CRYOMICROSCOPY ; PARTICLES ; EPITOPES ; INFECTION ; CARRIER ; PROTEIN
摘要	To prepare the dominant multiepitope fusion antigen ROP2-SAG1 (RSmultiepitope) from Toxoplasma gondii in a prokaryotic system, the major immunodominant region (MIR) of the human hepatitis B virus core antigen (HBcAg(MIR)) was used as a delivery vector. The gene encoding the RSmultiepitope was inserted into HBcAg(MIR), and rHBcAg(MIR)-RSmultiepitope was prepared, purified, and administered to BALB/c mice through intradermal injection. An indirect enzyme-linked immunosorbent assay analysis based on a multiepitope peptide facilitated the specific differentiation of sera obtained from mice immunized with the rHBcAg(MIR)RSmultiepitope protein, and high titers (greater than 1:6,400) of specific anti-RSmultiepitope antibodies were obtained. Immunized splenocytes demonstrated enhanced IFN-gamma production. Based on these results, the HBcAg(MIR) vector is easily applied in vitro for targeting the RSmultiepitope and efficiently presents this target epitope for the induction of significant humoral and cellular immune responses. This study offers a novel strategy for the design of a target epitope delivery system for a toxoplasmosis vaccine.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81371668]
出版者	MARY ANN LIEBERT, INC
出版地	NEW ROCHELLE
ISSN	0882-8245
EISSN	1557-8976
卷号	30 期号:7 页码:508-515
DOI	10.1089/vim.2016.0135
页数	8
WOS类目	Immunology ; Virology
WOS研究方向	Immunology ; Virology
WOS记录号	WOS:000410787100006
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	28436740
SCOPUSEID	2-s2.0-85058760417
通讯作者地址	[Zhang, Lifang;Li, Wenshu]Wenzhou Med Univ, Dept Microbiol & Immunol, Wenzhou 325035, Zhejiang, Peoples R China.
Scopus学科分类	Immunology;Molecular Medicine;Virology
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/1082
专题	检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系
通讯作者	Zhang, Lifang; Li, Wenshu
作者单位	Wenzhou Med Univ, Dept Microbiol & Immunol, Wenzhou 325035, Zhejiang, Peoples R China
第一作者单位	温州医科大学
通讯作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Wang, Wenhuan,Feng, Fangfang,Lv, Jinhui,et al. Major Immunodominant Region of Hepatitis B Virus Core Antigen as a Delivery Vector to Improve the Immunogenicity of the Fusion Antigen ROP2-SAG1 Multiepitope from Toxoplasma gondii in Mice[J]. VIRAL IMMUNOLOGY,2017,30(7):508-515.
APA	Wang, Wenhuan., Feng, Fangfang., Lv, Jinhui., Xie, Zixin., Chen, Jun., ... & Li, Wenshu. (2017). Major Immunodominant Region of Hepatitis B Virus Core Antigen as a Delivery Vector to Improve the Immunogenicity of the Fusion Antigen ROP2-SAG1 Multiepitope from Toxoplasma gondii in Mice. VIRAL IMMUNOLOGY, 30(7), 508-515.
MLA	Wang, Wenhuan,et al."Major Immunodominant Region of Hepatitis B Virus Core Antigen as a Delivery Vector to Improve the Immunogenicity of the Fusion Antigen ROP2-SAG1 Multiepitope from Toxoplasma gondii in Mice".VIRAL IMMUNOLOGY 30.7(2017):508-515.