A panel of six-circulating miRNA signature in serum and its potential diagnostic value in colorectal cancer

doi:10.1016/j.lfs.2020.118226

科研成果详情

题名	A panel of six-circulating miRNA signature in serum and its potential diagnostic value in colorectal cancer
作者	Maminezhad, Hamidreza 1; Ghanadian, Sharareh 2; Pakravan, Katayoon 3; Razmara, Ehsan 4; Rouhollah, Fatemeh 5; Mossahebi-Mohammadi, Majid 6; Babashah, Sadegh 3
发表日期	2020-10-01
发表期刊	LIFE SCIENCES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Colorectal carcinoma Exosomes Circulating miRNA Biomarker Diagnosis
其他关键词	REAL-TIME PCR ; LET-7A MICRORNA ; METASTASIS ; BIOMARKERS ; EXOSOMES ; MIR-21
摘要	Aim: Colorectal carcinoma (CRC) is one of the most prevalent cancers throughout the world. Circulating serum-derived microRNAs (miRNAs, miRs) can be used as non-invasive biomarkers for CRC diagnosis. This study aimed to identify a panel of six serum exosomal miRNAs as novel diagnostic biomarkers for CRC. Main methods: Exosomes were isolated and characterized from the conditioned media of the human colon adenocarcinoma cells (HCT-116 and Caco2). Sera were isolated from peripheral blood of 45 CRC and also 45 healthy individuals. The expression levels and diagnostic value of candidate circulating miRNAs (miR-19a, miR-20a, miR-150, miR-143, miR-145, and let-7a) were measured through quantitative real-time PCR. Receiver operating characteristic (ROC) curves were applied to evaluate the diagnostic accuracy of selected miRNAs. The association of candidate miRNAs and clinicopathological characteristics e.g. tumor node metastasis (TNM) staging and lymph node metastasis (LNM) were further evaluated. Key findings: Circulating serum miR-19a, miR-20a, miR-150, and let-7a were significantly up-regulated in CRC patients, while miR-143 and miR-145 showed a significant down-regulation. The higher levels of miR-143 and miR-145 in patients with TNM stage I-II were detected, whereas miR-19a, miR-20a, miR-150, and let-7a were highly expressed in TNM stage III. The expression levels of miR-19a, miR-20a, and miR-150 were positively correlated with LNM status, while the expression levels of miR-143 and miR-145 were lower in patients with LNM. Area under the ROC curves of miR-19a, miR-20a, miR-150, miR-143, miR-145, and let-7a were 0.87, 0.83, 0.75, 0.76, 0.78 and 0.71, respectively. Significance: We established a panel of six-circulating miRNA signature (i.e. miR-19a, miR-20a, miR-143, miR-145, miR-150, and let-7a) in serum as a non-invasive biomarker for CRC diagnosis. These findings confirm that serum-derived miRNAs have a strong potential to be a diagnostic biomarker for patients with CRC.
出版者	PERGAMON-ELSEVIER SCIENCE LTD
出版地	OXFORD
ISSN	0024-3205
EISSN	1879-0631
卷号	258 页码:118226
DOI	10.1016/j.lfs.2020.118226
页数	10
WOS类目	Medicine, Research & Experimental ; Pharmacology & Pharmacy
WOS研究方向	Research & Experimental Medicine ; Pharmacology & Pharmacy
WOS记录号	WOS:000575172000003
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	32771555
SCOPUSEID	2-s2.0-85089189828
通讯作者地址	[Mossahebi-Mohammadi, Majid]School of Pharmaceutical Sciences and International Collaborative Center on Growth Factor Research,Wenzhou Medical University,Wenzhou,China ; [Babashah, Sadegh]Department of Molecular Genetics,Faculty of Biological Sciences,Tarbiat Modares University,P.O. Box: 14115-154,Tehran,Iran
Scopus学科分类	Biochemistry, Genetics and Molecular Biology (all);Pharmacology, Toxicology and Pharmaceutics (all)
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/10745
专题	药学院（分析测试中心）卓越中心_国际生长因子研究院
通讯作者	Mossahebi-Mohammadi, Majid; Babashah, Sadegh
作者单位	1.Department of Medicine,School of Medicine,Tehran University of Medical Sciences,Tehran,Iran; 2.Department of Microbiology,Faculty of Advanced Sciences and Technology,Tehran Medical Sciences of Islamic Azad University,Tehran,Iran; 3.Department of Molecular Genetics,Faculty of Biological Sciences,Tarbiat Modares University,Tehran,Iran; 4.Department of Medical Genetics,Faculty of Medical Sciences,Tarbiat Modares University,Tehran,Iran; 5.Department of Cellular and Molecular Sciences,Faculty of Advanced Sciences and Technology,Tehran Medical Sciences,Islamic Azad University,Tehran,Iran; 6.School of Pharmaceutical Sciences and International Collaborative Center on Growth Factor Research,Wenzhou Medical University,Wenzhou,China
通讯作者单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Maminezhad, Hamidreza,Ghanadian, Sharareh,Pakravan, Katayoon,et al. A panel of six-circulating miRNA signature in serum and its potential diagnostic value in colorectal cancer[J]. LIFE SCIENCES,2020,258:118226.
APA	Maminezhad, Hamidreza., Ghanadian, Sharareh., Pakravan, Katayoon., Razmara, Ehsan., Rouhollah, Fatemeh., ... & Babashah, Sadegh. (2020). A panel of six-circulating miRNA signature in serum and its potential diagnostic value in colorectal cancer. LIFE SCIENCES, 258, 118226.
MLA	Maminezhad, Hamidreza,et al."A panel of six-circulating miRNA signature in serum and its potential diagnostic value in colorectal cancer".LIFE SCIENCES 258(2020):118226.