Attenuation of Experimental Autoimmune Hepatitis in Mice with Bone Mesenchymal Stem Cell-Derived Exosomes Carrying MicroRNA-223-3p

doi:10.14348/molcells.2019.2283

科研成果详情

题名	Attenuation of Experimental Autoimmune Hepatitis in Mice with Bone Mesenchymal Stem Cell-Derived Exosomes Carrying MicroRNA-223-3p
作者	Lu, Feng-Bin 1,4; Chen, Da-Zhi 2; Chen, Lu1; Hu, En-De 1; Wu, Jin-Lu 1; Li, Hui 1; Gong, Yue-Wen 3; Lin, Zhuo1; Wang, Xiao-Dong 1; Li, Ji 1; Jin, Xiao-Ya1; Xu, Lan-Man1,5; Chen, Yong-Ping1
发表日期	2019-12
发表期刊	MOLECULES AND CELLS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	autoimmune liver disease exosomes immunomodulatory mesenchymal stromal cells
其他关键词	LIVER-INJURY ; STROMAL CELLS ; MICROVESICLES ; ACTIVATION ; RESPONSES ; PATHWAYS
摘要	MicroRNA- 223-3p (miR-223-3p) is one of the potential microRNAs that have been shown to alleviate inflammatory responses in pre-clinical investigations and is highly encased in exosomes derived from bone mesenchymal stem cells (MSC-exosomes). MSC-exosomes are able to function as carriers to deliver microRNAs into cells. Autoimmune hepatitis is one of the challenging liver diseases with no effective treatment other than steroid hormones. Here, we examined whether MSC-exosomes can transfer miR-223-3p to treat autoimmune hepatitis in an experimental model. We found that MSC-exosomes were successfully incorporated with miR-223-3p and delivered miR-223-3p into macrophages. Moreover, there was no toxic effect of exosomes on the macrophages. Furthermore, treatments of either exosomes or exosomes with miR-223-3p successfully attenuated inflammatory responses in the liver of autoimmune hepatitis and inflammatory cytokine release in both the liver and macrophages. The mechanism may be related to the regulation of miR-223-3p level and STAT3 expression in the liver and macrophages. These results suggest that MSC-exosomes can be used to deliver miR-223-3p for the treatment of autoimmune hepatitis.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81570514, 81600466, 81770585]; Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [LY15H030017, LQ15H030006]; Medical and Health Technology projects of Zhejiang Province [2020KY276]; Medical Award Fund, Beijing, China [YJHYXKYJJ-162]; Innovation Team of the Early Warning and Intervention [C20150005]; National Science and Technology Major Project, China [2017ZX10202201, 2017ZX10203201, 2018ZX10725506-001]
出版者	KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
出版地	SEOUL
ISSN	1016-8478
EISSN	0219-1032
卷号	42 期号:12 页码:906-918
DOI	10.14348/molcells.2019.2283
页数	13
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000504876600010
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	31826604
PMC记录号	PMC6939658
Scopus记录号	2-s2.0-85077349262
通讯作者地址	[Chen, Yong-Ping]Department of Infectious Diseases,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang Provincial Key Laboratory for Accurate Diagnosis and Treatment of Chronic Liver Diseases,Hepatology Institute of Wenzhou Medical University,Wenzhou Key Laboratory of Hepatology,Wenzhou,325000,China
scopus学科分类	Molecular Biology;Cell Biology
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/10533
专题	第一临床医学院（信息与工程学院）、附属第一医院_精准医学中心实验室附属第一医院
通讯作者	Chen, Yong-Ping
作者单位	1.Department of Infectious Diseases,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang Provincial Key Laboratory for Accurate Diagnosis and Treatment of Chronic Liver Diseases,Hepatology Institute of Wenzhou Medical University,Wenzhou Key Laboratory of Hepatology,Wenzhou,325000,China; 2.Department of Gastroenterology,The First Hospital of Peking University,Beijing,100034,China; 3.College of Pharmacy,Rady Faculty of Health Sciences,University of Manitoba,Winnipeg,R3T 2N2,Canada; 4.Department of Infectious Diseases,Tongde Hospital of Zhejiang Province,Hangzhou,310000,China; 5.Department of Infectious Diseases and Liver Diseases,Ningbo Medical Center Lihuili Hospital,Affiliated Hospital of Ningbo University,Ningbo,315040,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Lu, Feng-Bin,Chen, Da-Zhi,Chen, Lu,et al. Attenuation of Experimental Autoimmune Hepatitis in Mice with Bone Mesenchymal Stem Cell-Derived Exosomes Carrying MicroRNA-223-3p[J]. MOLECULES AND CELLS,2019,42(12):906-918.
APA	Lu, Feng-Bin., Chen, Da-Zhi., Chen, Lu., Hu, En-De., Wu, Jin-Lu., ... & Chen, Yong-Ping. (2019). Attenuation of Experimental Autoimmune Hepatitis in Mice with Bone Mesenchymal Stem Cell-Derived Exosomes Carrying MicroRNA-223-3p. MOLECULES AND CELLS, 42(12), 906-918.
MLA	Lu, Feng-Bin,et al."Attenuation of Experimental Autoimmune Hepatitis in Mice with Bone Mesenchymal Stem Cell-Derived Exosomes Carrying MicroRNA-223-3p".MOLECULES AND CELLS 42.12(2019):906-918.