Pressure overload by suprarenal aortic constriction in mice leads to left ventricular hypertrophy without c-Kit expression in cardiomyocytes

doi:10.1038/s41598-020-72273-3

科研成果详情

题名	Pressure overload by suprarenal aortic constriction in mice leads to left ventricular hypertrophy without c-Kit expression in cardiomyocytes
作者	Nicks, Amy M.1,2; Kesteven, Scott H.1,2; Li, Ming 1,4; Wu, Jianxin 1; Chan, Andrea Y.1; Naqvi, Nawazish 3; Husain, Ahsan 3; Feneley, Michael P.1,2; Smith, Nicola J.1,2; Iismaa, Siiri E.1,2; Graham, Robert M.1,2
发表日期	2020-09-18
发表期刊	SCIENTIFIC REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	CARDIAC STEM-CELLS ; CORONARY-ARTERIES ; HEART-FAILURE ; ANIMAL-MODELS ; C-KIT(+) CELLS ; HYPERTENSION ; REASSESSMENT ; REGENERATION ; DYSFUNCTION ; SUFFICIENT
摘要	Animal models of pressure overload are valuable for understanding hypertensive heart disease. We characterised a surgical model of pressure overload-induced hypertrophy in C57BL/6J mice produced by suprarenal aortic constriction (SAC). Compared to sham controls, at one week post-SAC systolic blood pressure was significantly elevated and left ventricular (LV) hypertrophy was evident by a 50% increase in the LV weight-to-tibia length ratio due to cardiomyocyte hypertrophy. As a result, LV end-diastolic wall thickness-to-chamber radius (h/R) ratio increased, consistent with the development of concentric hypertrophy. LV wall thickening was not sufficient to normalise LV wall stress, which also increased, resulting in LV systolic dysfunction with reductions in ejection fraction and fractional shortening, but no evidence of heart failure. Pathological LV remodelling was evident by the re-expression of fetal genes and coronary artery perivascular fibrosis, with ischaemia indicated by enhanced cardiomyocyte Hif1a expression. The expression of stem cell factor receptor, c-Kit, was low basally in cardiomyocytes and did not change following the development of robust hypertrophy, suggesting there is no role for cardiomyocyte c-Kit signalling in pathological LV remodelling following pressure overload.
资助项目	National Health and Medical Research Council of AustraliaNational Health and Medical Research Council of Australia [573732, 1074386]; National Heart Foundation of AustraliaNational Heart Foundation of Australia [G10S 5148]; Stem Cells Australia-the Australian Research Council Special Research Initiative in Stem Cell ScienceAustralian Research Council [SR110001002]; Leducq Transatlantic Network of Excellence in Cardiovascular Research grant; National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81670336]; R.T. Hall Trust grant
出版者	NATURE RESEARCH
出版地	BERLIN
ISSN	2045-2322
卷号	10 期号:1 页码:15318
DOI	10.1038/s41598-020-72273-3
页数	12
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000573751100002
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	32948799
PMC记录号	PMC7501855
SCOPUSEID	2-s2.0-85091098317
通讯作者地址	[Graham, Robert M.]Division of Molecular Cardiology and Biophysics,Victor Chang Cardiac Research Institute,405 Liverpool Street, Darlinghurst,Sydney,2010,Australia
Scopus学科分类	Multidisciplinary
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/10305
专题	基础医学院（机能实验教学中心）_基础医学研究中心_心脏再生医学研究所
通讯作者	Graham, Robert M.
作者单位	1.Division of Molecular Cardiology and Biophysics,Victor Chang Cardiac Research Institute,405 Liverpool Street, Darlinghurst,Sydney,2010,Australia; 2.St Vincent’s Clinical School,University of New South Wales,Sydney,2052,Australia; 3.Department of Medicine,Emory University,Atlanta,30322,United States; 4.Cardiac Regeneration Research Institute,Wenzhou Medical University,Wenzhou,325035,China
推荐引用方式 GB/T 7714	Nicks, Amy M.,Kesteven, Scott H.,Li, Ming,et al. Pressure overload by suprarenal aortic constriction in mice leads to left ventricular hypertrophy without c-Kit expression in cardiomyocytes[J]. SCIENTIFIC REPORTS,2020,10(1):15318.
APA	Nicks, Amy M.., Kesteven, Scott H.., Li, Ming., Wu, Jianxin., Chan, Andrea Y.., ... & Graham, Robert M.. (2020). Pressure overload by suprarenal aortic constriction in mice leads to left ventricular hypertrophy without c-Kit expression in cardiomyocytes. SCIENTIFIC REPORTS, 10(1), 15318.
MLA	Nicks, Amy M.,et al."Pressure overload by suprarenal aortic constriction in mice leads to left ventricular hypertrophy without c-Kit expression in cardiomyocytes".SCIENTIFIC REPORTS 10.1(2020):15318.