Unloading the infarcted heart affect MMPs-TIMPs axis in a rat cardiac heterotopic transplantation model

doi:10.1007/s11033-011-0736-z

科研成果详情

题名	Unloading the infarcted heart affect MMPs-TIMPs axis in a rat cardiac heterotopic transplantation model
作者	Wang, Wei-jian5,6,7,8; Meng, Zi-li 4,5,6,7; Mo, Yun-chang 8; Liu, Jun-wei 8; Sun, Cheng-chao3; Hu, Sheng-shou 5,6,7; Zhang, Hao 1,2,5,6,7
发表日期	2012-01
发表期刊	MOLECULAR BIOLOGY REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Animal model Experimental surgery Left ventricular assist device Myocardial infarction Myocardial remodeling
其他关键词	ASSIST DEVICE SUPPORT ; MATRIX METALLOPROTEINASES ; MYOCARDIAL-INFARCTION ; DILATED CARDIOMYOPATHY ; EXTRACELLULAR-MATRIX ; BONE-MARROW ; FAILURE ; EXPRESSION ; INHIBITION ; MOLECULES
摘要	Ventricular assist devices may function as a bridge to recovery or heart transplantation, however, little is known about its mechanisms. This study examined the role of matrix metalloproteinases (MMP)-tissue inhibitors of metalloproteinases (TIMP) axis in the process of recovery after unloading in a rat ischemic-induce heart failure (HF) model. Myocardial infarction model was created with the coronary artery ligation. The infarcted rats hearts were unloaded by heterotopic cardiac transplantation (n = 14). 2 weeks later, the function of normal and infarcted hearts with or without loading was evaluated by Langendorff perfusion model. The hearts were then harvested and prepared for the study of expression of MMPs and TIMPs. Developed pressure in the unloading group was higher than the loading group (P = 0.0074). Unloading increased the ratio of TIMP-1-MMP-1(1.38 +/- A 0.11 vs. 0.76 +/- A 0.09, P < 0.05), TIMP-2-MMP-2 (1.06 +/- A 0.10 vs. 0.33 +/- A 0.07, P < 0.01), TIMP-3-MMP-9(1.07 +/- A 0.08 vs. 0.59 +/- A 0.06, P < 0.05). Although MMP-1, 2, 9 were downregulated (P < 0.01, 0.01, 0.05, respectively), TIMP-2 and TIMP-3 upregulated (P < 0.01, 0.05, respectively), MMP-7 and TIMP-1 was not affected significantly. The infarcted cardiac function could be improved by unloading. It was attributed to downregulation of MMP-1, 2 and 9, and upregulation of TIMP-2 and -3, and furthermore, the ratio of TIMPs to MMPs was increased, which might be more sensitive than sole MMPs or TIMPs for the judgment of myocardial matrix homeostasis.
资助项目	Fok Ying Tung Education FoundationFok Ying Tung Education Foundation [121041]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81070099]
出版者	SPRINGER
出版地	DORDRECHT
ISSN	0301-4851
EISSN	1573-4978
卷号	39 期号:1 页码:277-283
DOI	10.1007/s11033-011-0736-z
页数	7
WOS类目	Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
WOS记录号	WOS:000297355700035
收录类别	SCIE ; PUBMED
URL	查看原文
Pubmed记录号	21559840
通讯作者地址	[Zhang, Hao]Res Ctr Cardiovasc Regenerat Med, Dept Cardiovasc Surg, Cardiovasc Inst, A 167 Beilishilu, Beijing 100037, Peoples R China.
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/10256
专题	附属第一医院附属第一医院_麻醉科
通讯作者	Zhang, Hao
作者单位	1.Res Ctr Cardiovasc Regenerat Med, Dept Cardiovasc Surg, Cardiovasc Inst, Beijing 100037, Peoples R China; 2.Fuwai Hosp, Beijing 100037, Peoples R China; 3.Wenzhou Med Coll, Affiliated Hosp 1, Dept Cardiac Thorac Surg, Wenzhou 325003, Zhejiang, Peoples R China; 4.Hebei Chest Hosp, Dept Cardiovasc Surg, Shijiazhuang 050041, Hebei Province, Peoples R China; 5.Chinese Acad Med Sci, Dept Surg, Beijing 10037, Peoples R China; 6.Chinese Acad Med Sci, Res Ctr Cardiac Regenerat Med, Cardiovasc Inst, Beijing 10037, Peoples R China; 7.Chinese Acad Med Sci, Fuwai Hosp, Beijing 10037, Peoples R China; 8.Wenzhou Med Coll, Dept Anesthesiol, Affiliated Hosp 1, Wenzhou 325003, Zhejiang, Peoples R China
第一作者单位	附属第一医院; 麻醉科
推荐引用方式 GB/T 7714	Wang, Wei-jian,Meng, Zi-li,Mo, Yun-chang,et al. Unloading the infarcted heart affect MMPs-TIMPs axis in a rat cardiac heterotopic transplantation model[J]. MOLECULAR BIOLOGY REPORTS,2012,39(1):277-283.
APA	Wang, Wei-jian., Meng, Zi-li., Mo, Yun-chang., Liu, Jun-wei., Sun, Cheng-chao., ... & Zhang, Hao. (2012). Unloading the infarcted heart affect MMPs-TIMPs axis in a rat cardiac heterotopic transplantation model. MOLECULAR BIOLOGY REPORTS, 39(1), 277-283.
MLA	Wang, Wei-jian,et al."Unloading the infarcted heart affect MMPs-TIMPs axis in a rat cardiac heterotopic transplantation model".MOLECULAR BIOLOGY REPORTS 39.1(2012):277-283.