Comparison of extraction methods for quantifying extracellular polymers of marine algae

科研成果详情

题名	Comparison of extraction methods for quantifying extracellular polymers of marine algae
作者	Ge, Liyun; Huang, Yonggang; Gao, Dexiang ; Deng, Huanhuan
著作类型	图书著作
出版日期	2013
出版地	Kuala Lumpur
摘要	Five extraction methods were used to extract extracellular polymers (EPS) from Platymonas subcordiformis. Results suggested that the extraction efficiencies of EPS decreased as followed: cation exchange resin (CER) > ultrasonic extraction> heating (80 °C) > EDTA > centrifugation. CER method was the most effective in the EPS extraction from P. subcordiformis with the highest amount of EPS and the lowest cell lysis, while the ultrasonic extraction method was not suitable to extract EPS for its obvious cell lysis. In addition, heating (80 °C) was used to investigate EPS formation of P. subcordiformis. Results showed EPS amount increased at the increased incubation time when the nutrients were enough whereas the ratio of EPS components remained unchanged in the growing cycle. (2013) Trans Tech Publications, Switzerland.
ISBN	97830378
语种	英语
出版单位	Applied Mechanics and Materials
文献类型	专著
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/101265
专题	公共卫生与管理学院_环境科学系附属第二医院
作者单位	1.Department of Environmental Science, Wenzhou Medical College, Wenzhou, Zhejiang, China 2.Reproductive Center, 2nd Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang, China
推荐引用方式 GB/T 7714	Ge, Liyun,Huang, Yonggang,Gao, Dexiang,et al. Comparison of extraction methods for quantifying extracellular polymers of marine algae[M]. Kuala Lumpur,2013.