An optimized yeast cell-free lysate system for in vitro translation of human virus mRNA

科研成果详情

题名	An optimized yeast cell-free lysate system for in vitro translation of human virus mRNA
作者	Wang, Xiao; Zhao, Liang ; Zhao, Kong-Nan
著作类型	图书著作
出版日期	2014
摘要	Yeast (Saccharomyces cerevisiae) as a model organism has long been established to study various aspects of eukaryotic cellular and molecular biology. Cell-free lysates prepared from different yeast strains have been used as a powerful tool to study eukaryotic protein expression in vitro. Recently, we established a yeast cell-free lysate system for in vitro translation long and short L1 capsid gene mRNAs of human papillomavirus type 58. We were able to significantly improve the translation efficiencies of the viral mRNAs in the established system by optimizing the concentrations of potassium and magnesium and controlling the physiological status of the yeast cells used for lysate preparation. We proved that a single specific amino acid can be rate limiting for translation of a target mRNA. Here, we describe the materials, method, and technique used for the development of an efficient yeast cell-free translation system. 2014 Springer Science+Business Media, LLC.
ISBN	97816270
语种	英语
出版单位	Methods in Molecular Biology
文献类型	专著
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/101252
专题	检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系
作者单位	1.Department of Pathology, Shandong University School of Medicine, Jinan, China 2.Division of Molecular Genetics and Development, Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia 3.UQ Centre for Clinical Research, University of Queensland, Herston, Brisbane, QLD, Australia 4.Institute of Molecular Virology and Immunology, Wenzhou Medical College, Wenzhou, Zhejiang, China
推荐引用方式 GB/T 7714	Wang, Xiao,Zhao, Liang,Zhao, Kong-Nan. An optimized yeast cell-free lysate system for in vitro translation of human virus mRNA[M],2014.