左旋精氨酸对兔肺缺血／再灌注损伤时Fas／FasL表达的影响

doi:10.3969/j.issn.1002-1949.2006.10.013

科研成果详情

题名	左旋精氨酸对兔肺缺血／再灌注损伤时Fas／FasL表达的影响
其他题名	Effect of L - arginine on expression of Fas/FasL mRNA in pulmonary injury induced by ischemia - reperfusion in rabbits
作者	王万铁1; 陈瑞杰2; 郝卯林1; 倪世容1; 王青 1; 王方岩1; 宋张娟1; 金可可1; 王卫1; 郑绿珍1; 汪洋 1
发表日期	2006
发表期刊	中国急救医学影响因子和分区
语种	中文
原始文献类型	期刊论文
关键词	肺再灌注损伤 Fas／F8s配体细胞凋亡左旋精氨酸
摘要	目的探讨左旋精氨酸对肺缺血/再灌注损伤(PIRI)时Fas/FasL表达的影响.方法采用在体兔单肺原位缺血/再灌注模型.实验兔30只,随机分为假手术对照组(C组)、肺缺血/再灌注组(I/R组)和肺缺血/再灌注加左旋精氨酸组(L-Arg组),每组10只.分别于再灌注3 h取左肺组织,观察Fas/Fas配体(Fas/FasL)mRNA定位表达、凋亡指数(AI)、肺组织湿干质量比(W/D)、肺损伤组织学定量评价指标(IQA)及光镜、电镜下的组织形态学改变.结果 L-Arg组Fas/FasLmRNA在肺小动脉内(外)膜、肺小静脉内膜、肺泡上皮及肺支气管上皮呈弱阳性表达,明显低于I/R组(P＜0.05);AI、W/D和IQA值显著低于I/R组(P＜0.01和P＜0.05);肺组织形态学异常改变不同程度减轻.结论左旋精氨酸可下调肺组织Fas/FasL mRNA的表达而减轻细胞凋亡,对PIRI发挥积极的防治作用.
其他摘要	Objective To investigate the effect of L-arginine (L-Arg) on expression of Fas/FasL mRNA during pulmonary ischemia and reperfusion injury (PIRI) in the rabbits. Methods Single lung ischemia and reperfusion animal model was used in vivo. The rabbits were randomly into three groups, control group (C, n = 10), ischemia/reperfusion group (I/R, n = 10) and I/R + L-arginine group(L-Arg, n = 10). Changes of several parameters which included apoptotic index (AI), wet to dry ratio of lung tissue weightf W/D) and index of quantitative assessment of histologic lung injury (IQA) were measured at 180 minutes after reperfusion in lung tissue. Meanwhile the location and expression of Fas/FasL mRNA were observed, lung tissue was prepared for light microscopic and electron microscopic observation at 60,180,300 minutes after reperfusion. Results As compared with group I/R, Fas/FasL mRNA slightly expressed in intima and extima of small pulmonary artery, alveoli, and bronchiole epithelia in L-Arg group. The values of AI, W/D and IQA showed significantly lower than that in I/R group at 180 minutes after reperfusion in lung tissue (P < 0. 01 and P < 0. 05). Meanwhile, abnormal changes of the lung tissue in morphologically were lessen markedly in L-Arg group. Conclusion L-arginine produces notable protective effects on PIRI in rabbits by inhibiting Fas/FasL mRNA express in lung tissue, decreasing apoptosis.
资助项目	浙江省教育厅科研计划项目 ; 浙江省温州市科技计划重点项目
ISSN	1002-1949
卷号	26 期号:10 页码:674-677
DOI	10.3969/j.issn.1002-1949.2006.10.013
页数	4
收录类别	CSCD ; 万方 ; PKU ; 北大核心 ; ISTIC
学科领域	细胞生物学 ; 医药、卫生 ; 基础医学
URL	查看原文
CSCD记录号	CSCD:2425210
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/100053
专题	基础医学院（机能实验教学中心）_机能学系_生理学第二临床医学院、附属第二医院、育英儿童医院
作者单位	1.温州医学院病理生理学教研室, 浙江, 温州, 325035 ; 2.温州医学院附属第二医院药剂科, 浙江, 温州, 325027
第一作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	王万铁,陈瑞杰,郝卯林,等. 左旋精氨酸对兔肺缺血／再灌注损伤时Fas／FasL表达的影响[J]. 中国急救医学,2006,26(10):674-677.
APA	王万铁., 陈瑞杰., 郝卯林., 倪世容., 王青., ... & 汪洋. (2006). 左旋精氨酸对兔肺缺血／再灌注损伤时Fas／FasL表达的影响. 中国急救医学, 26(10), 674-677.
MLA	王万铁,et al."左旋精氨酸对兔肺缺血／再灌注损伤时Fas／FasL表达的影响".中国急救医学 26.10(2006):674-677.