IGF-1R stimulation alters microglial polarization via TLR4/NF-kappa B pathway after cerebral hemorrhage in mice

doi:10.1016/j.brainresbull.2020.08.026

科研成果详情

题名	IGF-1R stimulation alters microglial polarization via TLR4/NF-kappa B pathway after cerebral hemorrhage in mice
作者	Sun, Zhezhe 1; Wu, Ke 2; Gu, Lei 3; Huang, Lijie4,5; Zhuge, Qichuan4,5; Yang, Su 5; Wang, Zhenzhong 6
发表日期	2020-11
发表期刊	BRAIN RESEARCH BULLETIN 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Intracerebral hemorrhage neural stem cells insulin-like growth factor-1 microglial polarization TLR4/NF-kappa B
其他关键词	GROWTH-FACTOR-I ; NEURAL STEM-CELLS ; TRANSECTED SPINAL-CORD ; INTRACEREBRAL HEMORRHAGE ; TRANSPLANTATION ; INHIBITION ; EXPRESSION ; PROTEIN ; ASTROCYTES ; STROKE
摘要	Intracerebral hemorrhage (ICH) is a common neurological disease, causing severe disability and even deaths. Stem cell transplantation has been increasingly used in stroke therapy, and neural stem cells (NSCs) are a good source for stem cell transplantation. However, the regulatory mechanism of NSCs remains unclear. In this study, we examined the impact of insulin-like growth factor-1 (IGF-1) secreted by NSCs on microglial polarization following ICH in adult C57BL/6 mice. Mouse models of ICH were established by collagenase injection. ICH mice received NSC transplantation 1 h after model establishment. Firstly, the changes of microglial polarization in cerebral tissues of ICH mice were detected by immunofluorescence and ELISA. Secondly, the molecular mechanism underlying the microglial polarization was evaluated repeatedly with the application of IGF-1R siRNA and IGF-1R-mediated inhibition. We assessed the brain water content and behavioral deficits of ICH mice 12, 24, 48, and 72 h after surgery. The survival of neurons in the brain was examined using Nissl staining and TUNEL staining at 72 h. The TLR4/NF-x13 pathway implicated in ICH-induced inflammation was profiled by Western blot analysis and immunohistochemistry. Finally, the changes in microglia after ICH in mice were re-examined under different doses of rhIGF-1. In summary, NSC transplantation changed microglial polarization in ICH mice. IGF-1R inhibition and knockdown reversed the therapeutic effect of NSCs, and rhIGF-1 had an anti-inflammatory effect. The results of this study suggest that IGF-1R stimulation is a potential target for stem cell-based treatment of cerebral hemorrhage and may attenuate inflammatory response in the brain after bleeding.
资助项目	Ningbo Natural Science Foundation, China [2018A610310]; Yuyao City Science and Technology Council Foundation, China [2018YZD01]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81771262, 81820108011]
出版者	PERGAMON-ELSEVIER SCIENCE LTD
出版地	OXFORD
ISSN	0361-9230
EISSN	1873-2747
卷号	164 页码:221-234
DOI	10.1016/j.brainresbull.2020.08.026
页数	14
WOS类目	Neurosciences
WOS研究方向	Neurosciences & Neurology
WOS记录号	WOS:000582395500022
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	32871240
SCOPUSEID	2-s2.0-85090422864
通讯作者地址	[Wang, Zhenzhong]Department of Neurosurgery,Yuyao People's Hospital,Ningbo,315000,China ; [Zhuge, Qichuan]Department of Neurosurgery,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research,Wenzhou Medical University,Wenzhou,325000,China ; [Yang, Su]First Affiliated Hospital of Wenzhou Medical University,Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research,Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Neuroscience (all)
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/10003
专题	附属第一医院第一临床医学院（信息与工程学院）、附属第一医院_老化与神经疾病重点实验室
通讯作者	Zhuge, Qichuan; Yang, Su; Wang, Zhenzhong
作者单位	1.Department of Cerebrovascular,The Affiliated Hospital of Medical School of Ningbo University,Ningbo,315000,China; 2.Zhejiang University,Hangzhou,310000,China; 3.Department of Rehabilitation,Ningbo Medical Center Lihuili Hospital,Ningbo,315000,China; 4.Department of Neurosurgery,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 5.Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 6.Department of Neurosurgery,Yuyao People's Hospital,Ningbo,315000,China
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
推荐引用方式 GB/T 7714	Sun, Zhezhe,Wu, Ke,Gu, Lei,et al. IGF-1R stimulation alters microglial polarization via TLR4/NF-kappa B pathway after cerebral hemorrhage in mice[J]. BRAIN RESEARCH BULLETIN,2020,164:221-234.
APA	Sun, Zhezhe., Wu, Ke., Gu, Lei., Huang, Lijie., Zhuge, Qichuan., ... & Wang, Zhenzhong. (2020). IGF-1R stimulation alters microglial polarization via TLR4/NF-kappa B pathway after cerebral hemorrhage in mice. BRAIN RESEARCH BULLETIN, 164, 221-234.
MLA	Sun, Zhezhe,et al."IGF-1R stimulation alters microglial polarization via TLR4/NF-kappa B pathway after cerebral hemorrhage in mice".BRAIN RESEARCH BULLETIN 164(2020):221-234.